Application
Immunofluorescence analysis of methanol-fixed HeLa, using Kinectin 1A antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Kinectin 1A antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE diluted at 1:1000Application
Kinectin 1A antibody [C2C3], C-term detects Kinectin 1A protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Kinectin 1A antibody [C2C3], C-term diluted at 1:500.| Product Name | Kinectin 1A antibody [C2C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Kinectin 1A (kinectin 1 (kinesin receptor))Various cellular organelles and vesicles are transported along the microtubules in the cytoplasm. Likewise, membrane recycling of the endoplasmic reticulum (ER), Golgi assembly at the microtubule organizing center, and alignment of lysosomes along microtubules are all related processes. The transport of organelles requires a special class of microtubule-associated proteins (MAPs). One of these is the molecular motor kinesin (see MIM 148760 and MIM 600025), an ATPase that moves vesicles unidirectionally toward the plus end of the microtubule. Another such MAP is kinectin, a large integral ER membrane protein. Antibodies directed against kinectin have been shown to inhibit its binding to kinesin.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Kinectin 1A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | PigPig (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CG1 antibody, KIAA0004 antibody, KNT antibody, MGC133337 antibody, MU-RMS-40.19 antibody, KTN1 antibody, CG-1 antigen antibody, kinesin receptor antibody, kinectin antibody, kinectin 1 (kinesin receptor) antibody |
|---|---|
| Molecular Weight(MW) | 156 kDa (note) (4) |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type II membrane protein |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Kinectin 1A antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Kinectin 1A antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE diluted at 1:1000
Application
Kinectin 1A antibody [C2C3], C-term detects Kinectin 1A protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Kinectin 1A antibody [C2C3], C-term diluted at 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
