Application
KIR2DL4 antibody detects KIR2DL4 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KIR2DL4 protein stained by KIR2DL4 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using KIR2DL4 antibody at 1:250 dilution.Application
Sample (30 ug of whole cell lysate) A: THP-1 10% SDS PAGE diluted at 1:1000| Product Name | KIR2DL4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to KIR2DL4 (killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 4)Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. This gene is one of the "framework" loci that is present on all haplotypes. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human KIR2DL4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus Monkey, ChimpanzeeRhesus Monkey (89%), Chimpanzee (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD158D antibody, G9P antibody, KIR103 antibody, KIR103AS antibody, KIR2DL4 antibody, natural killer cell inhibitory receptor antibody, CD158 antigen-like family member D antibody, killer Ig receptor antibody, NK cell receptor antibody, killer cell immunogl |
|---|---|
| Molecular Weight(MW) | 41 kDa (note) (3) |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
Application
KIR2DL4 antibody detects KIR2DL4 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KIR2DL4 protein stained by KIR2DL4 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using KIR2DL4 antibody at 1:250 dilution.
Application
Sample (30 ug of whole cell lysate) A: THP-1 10% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
