Application
KIR2DL4 Antibody (C-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with KIR2DL4 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of KIR2DL4 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). KIR2DL4 (arrow) was detected using the purified Pab.| Product Name | KIR2DL4 antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to KIR2DL4 (C-term)Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. This gene is one of the "framework" loci that is present on all haplotypes. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 302~332 amino acids from the C-terminal region of human KIR2DL4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000 | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Killer cell immunoglobulinlike receptor2DL4, CD158 antigen-like family member D, CD_antigen=CD158d, CD158 antigenlike family member D, Killer cell inhibitory receptor 103AS, KIR103AS, G9P, Killer cell immunoglobulin-like receptor 2DL4, Flags: Precursor, KI |
|---|---|
| Target | KIR2DL4 |
| Molecular Weight(MW) | 41.528 kDa (note) |
Application
KIR2DL4 Antibody (C-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with KIR2DL4 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of KIR2DL4 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). KIR2DL4 (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
