Application
Immunofluorescence analysis of methanol-fixed A431, using KIR2DS2 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using KIR2DS2 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE diluted at 1:1000| Product Name | KIR2DS2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to KIR2DS2 (killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 2)Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human KIR2DS2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 183ActI antibody, CD158J antibody, CD158b antibody, MGC120018 antibody, MGC120020 antibody, MGC149477 antibody, MGC149478 antibody, NKAT5 antibody, cl-49 antibody, KIR2DS2 antibody, natural killer cell inhibitory receptor antibody, killer-cell Ig-like rece |
|---|---|
| Molecular Weight(MW) | 34 kDa (note) (3) |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
Application
Immunofluorescence analysis of methanol-fixed A431, using KIR2DS2 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using KIR2DS2 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
