Application
KLF10 antibody immunoprecipitates KLF10 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of KLF10 protein by 2 μg of KLF10 antibody 7.5% SDS-PAGE The immunoprecipitated KLF10 protein was detected by KLF10 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
KLF10 antibody detects KLF10 protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg HeLa nuclear lysate/extract 10% SDS-PAGE KLF10 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
KLF10 antibody detects KLF10 protein by western blot analysis. A. 30 µg 293T B. 30 µg of eGFP-human KLF10-transfected 293T cells 7.5% SDS-PAGE KLF10 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | KLF10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to KLF10/TIEG1 (Kruppel-like factor 10) |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human KLF10/TIEG1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EGRA antibody, TIEG antibody, TIEG1 antibody, KLF10 antibody, EGR-alpha antibody, Krueppel-like factor 10 antibody, TGFB inducible early growth response antibody, TGFB-inducible early growth response protein 1 antibody, transforming growth factor-beta-indu |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
KLF10 antibody immunoprecipitates KLF10 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of KLF10 protein by 2 μg of KLF10 antibody 7.5% SDS-PAGE The immunoprecipitated KLF10 protein was detected by KLF10 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
KLF10 antibody detects KLF10 protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg HeLa nuclear lysate/extract 10% SDS-PAGE KLF10 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
KLF10 antibody detects KLF10 protein by western blot analysis. A. 30 µg 293T B. 30 µg of eGFP-human KLF10-transfected 293T cells 7.5% SDS-PAGE KLF10 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HeLa nuclear , eGFP-human KLF10-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
