Polyclonal Anti - FKLFA novel Kruppel-type zinc finger protein, FKLF, that was cloned from globin-expressing human fetal erythroid cells. FKLF acts as a transcriptional activator of embryonic and fetal beta-globin genes.
Immunogen
Synthetic peptide: ERKRHDSERST, corresponding to amino acids 26-36 of Human FKLF.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
Western blot – Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Reacts with residues 26-36 [ERKRHDSERST] of the FKLF protein. Tested in human fetal liver tissue and the K562 erythroid cell line. Low levels of protein seen in other adult and fetal tissues or non-erythroid cell lines.
Application Notes
Western blot – Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.