Application
KLF4 antibody detects KLF4 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: KLF4 protein stained by KLF4 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
KLF4 antibody detects KLF4 protein at nucleus on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. KLF4 antibody dilution: 1:500.Application
KLF4 antibody detects KLF4 protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. KLF4 antibody dilution: 1:500.| Product Name | KLF4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to KLF4 (Kruppel-like factor 4 (gut))Transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human KLF4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Cat, Pig, Sheep, Rhesus Monkey, BovineRat (87%), Cat (91%), Pig (93%), Sheep (88%), Rhesus Monkey (98%), Bovine (88%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EZF antibody, GKLF antibody, KLF4 antibody, endothelial Kruppel-like zinc finger protein antibody, Krueppel-like factor 4 antibody, gut-enriched krueppel-like factor antibody, epithelial zinc finger protein EZF antibody, Kruppel-like factor 4 (gut) antibod |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
KLF4 antibody detects KLF4 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: KLF4 protein stained by KLF4 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
KLF4 antibody detects KLF4 protein at nucleus on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. KLF4 antibody dilution: 1:500.
Application
KLF4 antibody detects KLF4 protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. KLF4 antibody dilution: 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
