Application
Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using KLHL13 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE diluted at 1:1000Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000| Product Name | KLHL13 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to KLHL13 (kelch-like 13 (Drosophila))This gene encodes a BTB and kelch domain containing protein and belongs to the kelch repeat domain containing superfamily of proteins. The encoded protein functions as an adaptor protein that complexes with Cullin 3 and other proteins to form the Cullin 3-based E3 ubiquitin-protein ligase complex. This complex is necessary for proper chromosome segregation and completion of cytokinesis. Alternate splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human KLHL13. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Chicken, BovineZebrafish (94%), Xenopus laevis (94%), Chicken (96%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BKLHD2 antibody, FLJ10262 antibody, MGC74791 antibody, KLHL13 antibody, kelch-like protein 13 antibody, BTB and kelch domain containing 2 antibody, kelch-like 13 (Drosophila) antibody |
|---|---|
| Molecular Weight(MW) | 74 kDa (note) (7) |
Application
Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using KLHL13 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE diluted at 1:1000
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
