Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KLHL2 monoclonal antibody.Application
HEK293T cells transfected with either RC205347 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-KLHL2 antibody, and then analyzed by flow cytometry.Application
Anti-KLHL2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with KLHL2Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLHL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLHL2.| Product Name | KLHL2 antibody [1G7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1G7] to KLHL2May play a role in organizing the actin cytoskeleton of the brain cells. |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1-100 and 494-593 of human KLHL2 (NP_009177) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 1G7 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 | |
| Species Reactivity | HumanDogMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ABP-KELCH, MAV, MAYVEN, KLHL2, ABPKELCH, ABP KELCH |
|---|---|
| Target | KLHL2 |
| Tissue Specificity | is specific for amino acids 1-100 and 494-593 of human KLHL2 (NP_009177). |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KLHL2 monoclonal antibody.
Application
HEK293T cells transfected with either RC205347 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-KLHL2 antibody, and then analyzed by flow cytometry.
Application
Anti-KLHL2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with KLHL2
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLHL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLHL2.| Application Notes | ICC/IF 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
