Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using KLHL21 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:1000| Product Name | KLHL21 antibody [C1C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to KLHL21 (kelch-like 21 (Drosophila))Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for efficient chromosome alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human KLHL21. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, BovineMouse (95%), Rat (94%), Xenopus laevis (80%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KIAA0469 antibody, MGC99635 antibody, KLHL21 antibody, kelch-like protein 21 antibody, kelch-like 21 (Drosophila) antibody |
|---|---|
| Molecular Weight(MW) | 67 kDa (note) (2) |
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using KLHL21 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:1000| Positive Control | A549 , HeLa , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
