Application
Immunofluorescence analysis of methanol-fixed HeLa, using KPNA5 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000| Product Name | KPNA5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to KPNA5 (importin subunit alpha-6)The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA5 protein belongs to the importin alpha protein family and is thought to be involved in NLS-dependent protein import into the nucleus. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human KPNA5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Rat, ZebrafishRat (96%), Zebrafish (83%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IPOA6 antibody, SRP6 antibody, KPNA5 antibody, importin alpha 6 antibody, karyopherin subunit alpha-5 antibody, importin subunit alpha-6 antibody, karyopherin alpha 5 (importin alpha 6) antibody |
|---|---|
| Molecular Weight(MW) | 60 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using KPNA5 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
