Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using KPNA4 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T B: HepG2 C: Molt-4 7.5% SDS PAGE diluted at 1:1500Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using QIP1 antibody at 1:200 dilution.Application
KPNA4 antibody [C3], C-term detects KPNA4 protein by Western blot analysis. A. 30 µg Rat2 whole cell lysate/extract 7.5 % SDS-PAGE KPNA4 antibody [C3], C-term dilution: 1:1000Application
KPNA4 antibody [C3], C-term detects KPNA4 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract 7.5 % SDS-PAGE KPNA4 antibody [C3], C-term dilution: 1:1000| Product Name | KPNA4 antibody [C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to KPNA4 (karyopherin alpha 4 (importin alpha 3))The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human KPNA4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Chimpanzee, BovineChimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IPOA3 antibody, MGC12217 antibody, MGC26703 antibody, QIP1 antibody, SRP3 antibody, KPNA4 antibody, importin alpha Q1 antibody, importin subunit alpha-4 antibody, karyopherin subunit alpha-4 antibody, karyopherin alpha 4 (importin alpha 3) antibody |
|---|---|
| Molecular Weight(MW) | 58 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using KPNA4 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T B: HepG2 C: Molt-4 7.5% SDS PAGE diluted at 1:1500
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using QIP1 antibody at 1:200 dilution.
Application
KPNA4 antibody [C3], C-term detects KPNA4 protein by Western blot analysis. A. 30 µg Rat2 whole cell lysate/extract 7.5 % SDS-PAGE KPNA4 antibody [C3], C-term dilution: 1:1000
Application
KPNA4 antibody [C3], C-term detects KPNA4 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract 7.5 % SDS-PAGE KPNA4 antibody [C3], C-term dilution: 1:1000| Positive Control | 293T , HeLa , HepG2 , Molt-4 , Raji , *A549 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
