Application
Ku70 antibody immunoprecipitates Ku70 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Ku70 protein by 2.5 μg of Ku70 antibody 7.5% SDS-PAGE The immunoprecipitated Ku70 protein was detected by Ku70 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Immunohistochemical analysis of paraffin-embedded D54 xenograft, using Ku70 antibody at 1:500 dilution.Application
Ku70 antibody detects Ku70 protein at nucleus by immunofluorescent analysis. Samples: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ku70 protein stained by Ku70 antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.Application
Ku70 antibody detects Ku70 protein at nucleus in human cervical cancer by immunohistochemical analysis. Sample: Paraffin-embedded human cervical cancer. Ku70 antibody diluted at 1:500.Application
Ku70 antibody detects Ku70 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku70 antibody diluted by 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Ku70 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Ku70 (X-ray repair complementing defective repair in Chinese hamster cells 6)The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Ku70. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Chimpanzee, BovineMouse (82%), Rat (83%), Pig (87%), Chimpanzee (100%), Bovine (87%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CTC75 antibody, CTCBF antibody, G22P1 antibody, KU70 antibody, ML8 antibody, TLAA antibody, XRCC6 antibody, CTC box-binding factor 75 kDa subunit antibody, ATP-dependent DNA helicase II, 70 kDa subunit antibody, ATP-dependent DNA helicase II 70 kDa subunit |
|---|---|
| Molecular Weight(MW) | 70 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
Ku70 antibody immunoprecipitates Ku70 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Ku70 protein by 2.5 μg of Ku70 antibody 7.5% SDS-PAGE The immunoprecipitated Ku70 protein was detected by Ku70 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Immunohistochemical analysis of paraffin-embedded D54 xenograft, using Ku70 antibody at 1:500 dilution.
Application
Ku70 antibody detects Ku70 protein at nucleus by immunofluorescent analysis. Samples: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ku70 protein stained by Ku70 antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
Application
Ku70 antibody detects Ku70 protein at nucleus in human cervical cancer by immunohistochemical analysis. Sample: Paraffin-embedded human cervical cancer. Ku70 antibody diluted at 1:500.
Application
Ku70 antibody detects Ku70 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku70 antibody diluted by 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
