Application
Ku80(XRCC5) antibody immunoprecipitates Ku80 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Ku80 protein by 2.5 μg of Ku80 antibody 7.5% SDS-PAGE The immunoprecipitated Ku80 protein was detected by Ku80 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using Ku80 (XRCC5) antibody at 1:100 dilution.Application
Ku80 antibody [N3C2], Internal detects XRCC5 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 7.5% SDS-PAGE Ku80 antibody [N3C2], Internal dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Ku80 antibody [N3C2], Internal detects Ku80 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ku80 protein stained by Ku80 antibody [N3C2], Internal diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.| Product Name | Ku80 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Ku80 (X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining))The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Ku80. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ39089 antibody, KARP-1 antibody, KARP1 antibody, KU80 antibody, KUB2 antibody, Ku86 antibody, NFIV antibody, XRCC5 antibody, ATP-dependent DNA helicase II 80 kDa subunit antibody, CTC85 antibody, TLAA antibody, Ku86 autoantigen related protein 1 antibod |
|---|---|
| Molecular Weight(MW) | 83 kDa (note) |
| Cellular Localization | Nucleus |
Application
Ku80(XRCC5) antibody immunoprecipitates Ku80 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Ku80 protein by 2.5 μg of Ku80 antibody 7.5% SDS-PAGE The immunoprecipitated Ku80 protein was detected by Ku80 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using Ku80 (XRCC5) antibody at 1:100 dilution.
Application
Ku80 antibody [N3C2], Internal detects XRCC5 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 7.5% SDS-PAGE Ku80 antibody [N3C2], Internal dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Ku80 antibody [N3C2], Internal detects Ku80 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ku80 protein stained by Ku80 antibody [N3C2], Internal diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.| Positive Control | 293T , A431 , HepG2 , A375 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
