Application
L1CAM (150kDa subunit) antibody detects L1CAM (150kDa subunit) protein at cell membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. L1CAM (150kDa subunit) antibody diluted at 1:250.Application
L1CAM (150kDa subunit) antibody detects L1CAM (150kDa subunit) protein by western blot analysis. A. 30 μg U87-MG whole cell lysate/extract B. 30 μg SK-N-SH whole cell lysate/extract | C. 30 μg IMR32 whole cell lysate/extract D. 30 μg SK-N-AS whole cell lysate/extract 5 % SDS-PAGE L1CAM (150kDa subunit) antibody dilution: 1:1000| Product Name | L1CAM (150kDa subunit) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to L1CAM (150kDa subunit) (L1 cell adhesion molecule)The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human L1CAM (150kDa subunit). The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rabbit, Rhesus Monkey, Chimpanzee, BovineMouse (81%), Rat (81%), Rabbit (85%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (86%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NCAM-L1 SPG1 HSAS N-CAM-L1 CAML1 MASA N-CAML1 S10 CD171 L1 cell adhesion molecule HSAS1 MIC5 L1CAM |
|---|---|
| Target | L1CAM (150kDa subunit) |
| Molecular Weight(MW) | 140 kDa (note) (4) |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
Application
L1CAM (150kDa subunit) antibody detects L1CAM (150kDa subunit) protein at cell membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. L1CAM (150kDa subunit) antibody diluted at 1:250.
Application
L1CAM (150kDa subunit) antibody detects L1CAM (150kDa subunit) protein by western blot analysis. A. 30 μg U87-MG whole cell lysate/extract B. 30 μg SK-N-SH whole cell lysate/extract | C. 30 μg IMR32 whole cell lysate/extract D. 30 μg SK-N-AS whole cell lysate/extract 5 % SDS-PAGE L1CAM (150kDa subunit) antibody dilution: 1:1000| Positive Control | U87-MG , SK-N-SH , IMR32 , SK-N-AS |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
