Application
L3MBTL antibody detects L3MBTL protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human L3MBTL-transfected 293T cells 7.5 % SDS-PAGE L3MBTL antibody dilution: 1:5000Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using L3MBTL antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Sample (30 ug of whole cell lysate) A: HeLa nucleus 7.5% SDS PAGE diluted at 1:1000| Product Name | L3MBTL antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to L3MBTL (l(3)mbt-like 1 (Drosophila))This gene encodes the homolog of a protein identified in Drosophila as a suppressor of malignant transformation of neuroblasts and ganglion-mother cells in the optic centers of the brain. This gene product is localized to condensed chromosomes in mitotic cells. Overexpression of this gene in a glioma cell line results in improper nuclear segregation and cytokinesis producing multinucleated cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human L3MBTL. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | MouseMouse (83%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp586P1522 antibody FLJ41181 antibody H-L(3)MBT antibody KIAA0681 antibody L3MBTL antibody dJ138B7.3 antibody L3MBTL1 antibody lethal (3) malignant brain tumor l(3) antibody lethal(3)malignant brain tumor-like protein 1 antibody l(3)mbt protein |
|---|---|
| Molecular Weight(MW) | 84 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
L3MBTL antibody detects L3MBTL protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human L3MBTL-transfected 293T cells 7.5 % SDS-PAGE L3MBTL antibody dilution: 1:5000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using L3MBTL antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Sample (30 ug of whole cell lysate) A: HeLa nucleus 7.5% SDS PAGE diluted at 1:1000| Positive Control | HeLa nucleus , human L3MBTL-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
