Application
WB analysis of nuclear extracts from HeLa cells using L3MBTL1 antibody at a dilution of 1:1,000 in TBS-Tween containing 5% skimmed milk.Application
Immunoprecipitation was performed on nuclear extracts from cells transfected with FLAG-tagged L3MBTL1 using L3MBTL1 antibody and a control IgG. The FLAG-tagged L3MBTL1 in the input (In), in the precipitated fraction (P) and in the supernatant (SN) was detected by WB using FLAG antibody.| Product Name | L3MBTL1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to L3MBTL1This gene represents a polycomb group gene. The encoded protein functions to regulate gene activity, likely via chromatin modification. The encoded protein may also be necessary for mitosis. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010] |
| Immunogen | Polyclonal antibody raised in rabbit against human L3MBTL1 (lethal 3 malignant brain tumor-like protein), using the N-terminal part of the protein. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IPWB |
| Western blotting (1:1,000), Immunoprecipitation (1 - 5 µg per IP): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | ZC2HC3 l(3)mbt-like 1 (Drosophila) dJ138B7.3 H-L(3)MBT L3MBTL |
|---|---|
| Target | L3MBTL1 |
Application
WB analysis of nuclear extracts from HeLa cells using L3MBTL1 antibody at a dilution of 1:1,000 in TBS-Tween containing 5% skimmed milk.
Application
Immunoprecipitation was performed on nuclear extracts from cells transfected with FLAG-tagged L3MBTL1 using L3MBTL1 antibody and a control IgG. The FLAG-tagged L3MBTL1 in the input (In), in the precipitated fraction (P) and in the supernatant (SN) was detected by WB using FLAG antibody.| Application Notes | Western blotting (1:1,000), Immunoprecipitation (1 - 5 µg per IP): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
