Application
L3MBTL2 antibody immunoprecipitates L3MBTL2 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of L3MBTL2 antibody The precipitated DNA was detected by PCR with primer set targeting to CDC7 gene locus.Application
L3MBTL2 antibody immunoprecipitates L3MBTL2 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of L3MBTL2 protein by 2.5 μg of L3MBTL2 antibody 5% SDS-PAGE The immunoprecipitated L3MBTL2 protein was detected by L3MBTL2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with L3MBTL2 antibody diluted at 1:500.Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with L3MBTL2 antibody diluted at 1:500.| Product Name | L3MBTL2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to L3MBTL2 (l(3)mbt-like 2 (Drosophila))Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Its association with a chromatin-remodeling complex suggests that it may contribute to prevent expression of genes that trigger the cell into mitosis. Binds to monomethylated and dimethylated 'Lys-20' on histone H4. Binds histone H3 peptides that are monomethylated or dimethylated on 'Lys-4', 'Lys-9' or 'Lys-27'. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human L3MBTL2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (87%), Bovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H-l(3)mbt-l antibody L3MBT antibody L3MBTL2 antibody H-l(3)mbt-like protein 2 antibody lethal(3)malignant brain tumor-like protein 2 antibody L(3)mbt-like protein 2 antibody l(3)mbt-like 2 (Drosophila) antibody |
|---|---|
| Molecular Weight(MW) | 79 kDa (note) |
| Cellular Localization | Nucleus |
Application
L3MBTL2 antibody immunoprecipitates L3MBTL2 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of L3MBTL2 antibody The precipitated DNA was detected by PCR with primer set targeting to CDC7 gene locus.
Application
L3MBTL2 antibody immunoprecipitates L3MBTL2 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of L3MBTL2 protein by 2.5 μg of L3MBTL2 antibody 5% SDS-PAGE The immunoprecipitated L3MBTL2 protein was detected by L3MBTL2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with L3MBTL2 antibody diluted at 1:500.
Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with L3MBTL2 antibody diluted at 1:500.| Positive Control | NT2D1 , mouse testis |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
