Application
Immunoprecipitation of Lamin B1 protein from 293T whole cell extracts using 5 μg of Lamin B1 antibody . Western blot analysis was performed using Lamin B1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Lamin B1 antibody detects Lamin B1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Lamin B1 antibody antibody at 1:250 dilution.| Product Name | Lamin B1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Lamin B1 (lamin B1)The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Lamin B1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, Rhesus Monkey, Chimpanzee, BovineMouse (98%), Rat (98%), Xenopus laevis (85%), Chicken (91%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ADLD antibody LMN antibody LMN2 antibody LMNB antibody MGC111419 antibody LMNB1 antibody lamin-B1 antibody lamin B1 antibody |
|---|---|
| Molecular Weight(MW) | 66 kDa (note) (2) |
| Cellular Localization | Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side |
Application
Immunoprecipitation of Lamin B1 protein from 293T whole cell extracts using 5 μg of Lamin B1 antibody . Western blot analysis was performed using Lamin B1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Lamin B1 antibody detects Lamin B1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Lamin B1 antibody antibody at 1:250 dilution.| Positive Control | 293T , A431 , HeLa , HepG2 , *Huh7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
