Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B2 antibody [N3C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: H1299 B: HeLa C: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Lamin B2 antibody [N3C2], Internal detects LMNB2 protein at on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. Lamin B2 antibody [N3C2], Internal dilution: 1:500.Application
Lamin B2 antibody [N3C2], Internal detects LMNB2 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract 7.5% SDS-PAGE Lamin B2 antibody [N3C2], Internal dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Lamin B2 antibody [N3C2], Internal immunoprecipitates Lamin B2 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of Lamin B2 protein expressing HepG2 cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of Lamin B2 by 3 μg of Lamin B2 antibody [N3C2], Internal 7.5% SDS-PAGE The immunoprecipitated Lamin B2 protein was detected by Lamin B2 antibody [N3C2], Internal diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.| Product Name | Lamin B2 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Lamin B2 (lamin B2)The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B2. This gene is in a head-to-tail orientation with the gene for the translocase of inner mitochondrial membrane 13 homolog gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Lamin B2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | BovineBovine (82%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LAMB2 antibody LMN2 antibody MGC2721 antibody LMNB2 antibody lamin-B2 antibody lamin B2 antibody |
|---|---|
| Molecular Weight(MW) | 68 kDa (note) |
| Cellular Localization | Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side |
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B2 antibody [N3C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: H1299 B: HeLa C: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Lamin B2 antibody [N3C2], Internal detects LMNB2 protein at on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. Lamin B2 antibody [N3C2], Internal dilution: 1:500.
Application
Lamin B2 antibody [N3C2], Internal detects LMNB2 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract 7.5% SDS-PAGE Lamin B2 antibody [N3C2], Internal dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Lamin B2 antibody [N3C2], Internal immunoprecipitates Lamin B2 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of Lamin B2 protein expressing HepG2 cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of Lamin B2 by 3 μg of Lamin B2 antibody [N3C2], Internal 7.5% SDS-PAGE The immunoprecipitated Lamin B2 protein was detected by Lamin B2 antibody [N3C2], Internal diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
