Application
Immunocytochemistry/Immunofluorescence: Laminin Antibody - Detection of Laminin (Green) in Hela cells using at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.Application
Immunohistochemistry: Laminin Antibody - Staining of rat spinal cord and dorsal root paraformaldehyde/paraffin-embedded tissue using . Pepsin antigen retrieval was performed on this tissue sample.Application
Laminin-1 (LN-1) protein from mouse EHS tumor and Laminin-2 (LN-2) protein from heart.| Product Name | Laminin 1 + 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Laminin 1+2Laminin antibodies are widely used to label blood vessels and basement membranes. |
| Immunogen | Full length native protein (purified) (Mouse EHS tumour). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PInhibitionWB |
| HC-P: 1/1000 - 1/5000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Immunostaining enhanced by antigen retrieval with pepsin, especially paraffin tissue.IHC-Fr: 1/1000 - 1/5000. Inhib: This product functionally inhibits laminin in mouse and rat. Not tested in human tissue.WB: 1/2000 - 1/10000. Predicted molecular weight: 200 and 400 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AA408497 Lama |
|---|---|
| Target | Laminin 1 + 2 |
| Tissue Specificity | This antibody is pan-specific and reacts well with all laminin isoforms tested: laminin-1 (alpha-1, beta-1, and gamma-1) and laminin-2 (alpha-2, beta-1, and gamma-1) . |
Application
Immunocytochemistry/Immunofluorescence: Laminin Antibody - Detection of Laminin (Green) in Hela cells using at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.
Application
Immunohistochemistry: Laminin Antibody - Staining of rat spinal cord and dorsal root paraformaldehyde/paraffin-embedded tissue using . Pepsin antigen retrieval was performed on this tissue sample.
Application
Laminin-1 (LN-1) protein from mouse EHS tumor and Laminin-2 (LN-2) protein from heart.| Positive Control | Basement membranes (blood vessels of any tissue, tumors and nerve) |
|---|---|
| Application Notes | HC-P: 1/1000 - 1/5000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Immunostaining enhanced by antigen retrieval with pepsin, especially paraffin tissue.IHC-Fr: 1/1000 - 1/5000. Inhib: This product functionally inhibits laminin in mouse and rat. Not tested in human tissue.WB: 1/2000 - 1/10000. Predicted molecular weight: 200 and 400 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For long-term storage, keep at -20 °C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | Precipitated IgG with 0.01% sodium azide |
