Application
Laminin alpha 4 antibody [C3], C-term detects Laminin alpha 4 protein at cytoplasm on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Laminin alpha 4 antibody [C3], C-term diluted at 1:500.| Product Name | Laminin alpha 4 antibody , C-term |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Laminin alpha 4 (laminin, alpha 4)Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the alpha chain isoform laminin, alpha 4. The domain structure of alpha 4 is similar to that of alpha 3, both of which resemble truncated versions of alpha 1 and alpha 2, in that approximately 1,200 residues at the N-terminus (domains IV, V and VI) have been lost. Laminin, alpha 4 contains the C-terminal G domain which distinguishes all alpha chains from the beta and gamma chains. The RNA analysis from adult and fetal tissues revealed developmental regulation of expression, however, the exact function of laminin, alpha 4 is not known. Tissue-specific utilization of alternative polyA-signal has been described in literature. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Laminin alpha 4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, BovineHuman (100%), Rat (97%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686D23145 antibody LAMA3 antibody LAMA4*-1 antibody LAMA4 antibody laminin alpha 4 chain antibody laminin subunit alpha-4 antibody laminin alpha 4 antibody |
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| Molecular Weight(MW) | 203 kDa (note) |
| Cellular Localization | Secreted , extracellular space , extracellular matrix , basement membrane |
Application
Laminin alpha 4 antibody [C3], C-term detects Laminin alpha 4 protein at cytoplasm on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Laminin alpha 4 antibody [C3], C-term diluted at 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
