Application
Immunofluorescence staining of LAMP1 (red) in HeLa cells.Application
Western blot analysis of LAMP1 was performed by loading 20 μg of HeLa cell lysate and 5 μl of Molecular Weight Protein Ladder per well onto a 4-12% Bis-Tris polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an anti-LAMP1 polyclonal antibody at a dilution of 1:500 for one hour at room temperature, washed in TBS-0.1%Tween-20, and probed with a goat anti-rabbit IgG-HRP secondary antibody.| Product Name | LAMP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to LAMP1The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis. [provided by RefSeq, Jul 2008] |
| Immunogen | Synthetic peptide corresponding to residues C K(407) R S H A G Y Q T I(416) of human LAMP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB: 1:250 - 1:1000 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LAMP1 LGP120 LAMPA lysosomal-associated membrane protein 1 |
|---|---|
| Target | LAMP1 |
Application
Immunofluorescence staining of LAMP1 (red) in HeLa cells.
Application
Western blot analysis of LAMP1 was performed by loading 20 μg of HeLa cell lysate and 5 μl of Molecular Weight Protein Ladder per well onto a 4-12% Bis-Tris polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an anti-LAMP1 polyclonal antibody at a dilution of 1:500 for one hour at room temperature, washed in TBS-0.1%Tween-20, and probed with a goat anti-rabbit IgG-HRP secondary antibody.| Application Notes | WB: 1:250 - 1:1000 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | 0.02 M tris containing 0.1 % sodium azide and 0.1 M glycine |
