WB
LAMP-1 Antibody (H4A3): Near-infrared Western blot analysis of LAMP-1 expression in whole cell lysates from MDA-MB-231 (A), K-562 (B), HT-1080 (C), and JAR (D) cells. Detection reagent used: m-IgGκ BP-CFL 680: sc-516180.WB
LAMP-1 Antibody (H4A3): Western blot analysis of LAMP-1 expression in whole cell lysates from HeLa (A), JAR (B), ECV304 (C), U-937 (D), Jurkat (E), and Ramos (F) cells.IHC
LAMP-1 Antibody (H4A3): Immunoperoxidase staining of formalin-fixed, paraffin-embedded human esophageal tissue shows cytoplasmic staining in squamous epithelial cells.ICC/IF
LAMP-1 Antibody (H4A3): Immunofluorescence staining of methanol-fixed HeLa cells shows cytoplasmic and cell membrane staining.FC
LAMP-1 Antibody (H4A3) PE:Flow cytometric (FCM) analysis of JAR cells. Black line histogram represents isotype control, normal mouse IgG1| Product Name | Mouse Monoclonal antibody to LAMP1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against adherent spleen cells of human origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:100-1:1000 ICC/IF:1:50-1:500 IHC:1:50-1:500 IP:1 µg per 1 x 106 cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LAMP1 |
|---|---|
| Gene Synonyms | LAMPA CD107a LGP120 |
| Gene Full Name | lysosome associated membrane protein 1 |
| Gene Summary | The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 120 kDa |
| Cellular Localization | Cell membrane,Endosome,Lysosome,Membrane |
WB
LAMP-1 Antibody (H4A3): Near-infrared Western blot analysis of LAMP-1 expression in whole cell lysates from MDA-MB-231 (A), K-562 (B), HT-1080 (C), and JAR (D) cells. Detection reagent used: m-IgGκ BP-CFL 680: sc-516180.
WB
LAMP-1 Antibody (H4A3): Western blot analysis of LAMP-1 expression in whole cell lysates from HeLa (A), JAR (B), ECV304 (C), U-937 (D), Jurkat (E), and Ramos (F) cells.
IHC
LAMP-1 Antibody (H4A3): Immunoperoxidase staining of formalin-fixed, paraffin-embedded human esophageal tissue shows cytoplasmic staining in squamous epithelial cells.
ICC/IF
LAMP-1 Antibody (H4A3): Immunofluorescence staining of methanol-fixed HeLa cells shows cytoplasmic and cell membrane staining.
FC
LAMP-1 Antibody (H4A3) PE:Flow cytometric (FCM) analysis of JAR cells. Black line histogram represents isotype control, normal mouse IgG1| Application Notes | WB:1:100-1:1000 ICC/IF:1:50-1:500 IHC:1:50-1:500 IP:1 µg per 1 x 106 cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
