Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using LAMP2 antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using LAMP2 antibody at 1:500 dilution.Application
LAMP2 antibody detects LAMP2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP2 antibody diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | LAMP2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LAMP2 (lysosomal-associated membrane protein 2)The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LAMP2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD107b antibody LAMPB antibody LGP110 antibody LAMP2 antibody CD107 antigen-like family member B antibody lysosome-associated membrane glycoprotein 2 antibody lysosome-associated membrane protein 2 antibody lysosomal-associated membrane protein 2 an |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) (3) |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein , Endosome membrane; Single-pass type I membrane protein , Lysosome membrane; Single-pass type I membrane protein |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using LAMP2 antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using LAMP2 antibody at 1:500 dilution.
Application
LAMP2 antibody detects LAMP2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP2 antibody diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A431 , HeLa , HepG2 , *F79 , *F123 , *ARPE19 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
