Application
Immunofluorescence analysis of methanol-fixed HeLa, using LAMP3 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | LAMP3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LAMP3 (lysosomal-associated membrane protein 3)Dendritic cells (DCs) are the most potent antigen-presenting cells. Immature DCs efficiently capture antigens and differentiate into interdigitating dendritic cells (IDCs) in lymphoid tissues that induce primary T-cell responses (summary by de Saint-Vis et al., 1998 [PubMed 9768752]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LAMP3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus Monkey, ChimpanzeeRhesus Monkey (94%), Chimpanzee (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD208 antibody DC LAMP antibody DC-LAMP antibody DCLAMP antibody LAMP antibody LAMP-3 antibody TSC403 antibody LAMP3 antibody DC-lysosome-associated membrane glycoprotein antibody lysosome-associated membrane glycoprotein 3 antibody lysosomal-ass |
|---|---|
| Molecular Weight(MW) | 44 kDa (note) |
| Cellular Localization | Lysosome membrane; Single-pass type I membrane protein |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using LAMP3 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HeLa , Molt-4 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
