| Product Name | LAP2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [6E10] to LAP2Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP1), and several isoforms of LAP2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP2 beta and the nucleoplasmic protein LAP2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure. |
| Immunogen | Synthetic peptide: GEQRKDVYVQLYLQHLTARNRP, corresponding to amino acids 29-50 of Human LAP2, common to all isoforms. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6E10 |
| Host Species | Mouse |
| Tested Applications | IHC-PWB |
| IHC-P: Use at an assay dependent dilution. WB: Use at a concentration of 2 - 4 μg/ml. Detects bands of approximately 76 (alpha), 50 (beta, singlet or doublet), 45 (epsilon), 42 (delta), 38 (gamma, singlet or doublet) and 25 kDa (zeta). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRatXenopusBovine |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | CMD1T LAP2 LEMD4 MGC61508 PRO0868 TP |
|---|---|
| Target | LAP2 |
| Cellular Localization | Nuclear and Secreted |
| Positive Control | Whole extract of cultured human chronic myelogenous leukemia K562 cells |
|---|---|
| Application Notes | IHC-P: Use at an assay dependent dilution. WB: Use at a concentration of 2 - 4 μg/ml. Detects bands of approximately 76 (alpha), 50 (beta, singlet or doublet), 45 (epsilon), 42 (delta), 38 (gamma, singlet or doublet) and 25 kDa (zeta). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
