Application
LARGE antibody detects LARGE protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. LARGE antibody dilution: 1:500.Application
LARGE antibody detects LARGE protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. LARGE antibody dilution: 1:500.Application
LARGE antibody detects LARGE protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with LARGE antibody at a dilution of 1:1000.| Product Name | LARGE antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LARGE (like-glycosyltransferase)This gene, which is one of the largest in the human genome, encodes a member of the N-acetylglucosaminyltransferase gene family. It encodes a glycosyltransferase which participates in glycosylation of alpha-dystroglycan, and may carry out the synthesis of glycoprotein and glycosphingolipid sugar chains. It may also be involved in the addition of a repeated disaccharide unit. Mutations in this gene cause MDC1D, a novel form of congenital muscular dystrophy with severe mental retardation and abnormal glycosylation of alpha-dystroglycan. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LARGE. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | MouseRat |
| Predicted Reactivity | Human, Rhesus Monkey, BovineHuman (100%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MDDGB6 MDC1D MDDGA6 like-glycosyltransferase |
|---|---|
| Target | LARGE |
| Molecular Weight(MW) | 88 kDa (note) (3) |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein |
Application
LARGE antibody detects LARGE protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. LARGE antibody dilution: 1:500.
Application
LARGE antibody detects LARGE protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. LARGE antibody dilution: 1:500.
Application
LARGE antibody detects LARGE protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with LARGE antibody at a dilution of 1:1000.| Positive Control | mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
