Application
Western blot analysis of MAP1LC3A in HeLa cell lysate with MAP1LC3A antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunohistochemistry of MAP1LC3A in human brain tissue with MAP1LC3A antibody at 5 ug/mL.Application
Immunofluorescence of MAP1LC3A in human brain tissue with MAP1LC3A antibody at 20 ug/mL.| Product Name | LC3A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LC3AMAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012] |
| Immunogen | MAP1LC3A antibody was raised against a 10 amino acid peptide near the amino terminus of human MAP1LC3A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| WB: 1 - 2 ug/ml. IHC-P: 5 ug/mL. IF: 20 ug/ml: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | microtubule-associated protein 1 light chain 3 alpha MAP1LC3A LC3 MAP1BLC3 MAP1ALC3 ATG8E |
|---|---|
| Target | LC3A |
| Tissue Specificity | MAP1LC3A antibody is human specific. At least two isoforms of MAP1LC3A are known to exist. MAP1LC3A antibody is predicted to not cross-react with MAP1LC3B or MAP1LC3C. |
Application
Western blot analysis of MAP1LC3A in HeLa cell lysate with MAP1LC3A antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunohistochemistry of MAP1LC3A in human brain tissue with MAP1LC3A antibody at 5 ug/mL.
Application
Immunofluorescence of MAP1LC3A in human brain tissue with MAP1LC3A antibody at 20 ug/mL.| Application Notes | WB: 1 - 2 ug/ml. IHC-P: 5 ug/mL. IF: 20 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
