Application
Immunofluorescence staining of methanol-fixed Hela cells using LC3A antibody .Application
Western blot analysis of extracts from Hela and 293 cells using LC3A antibody .| Product Name | LC3A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LC3AProbably involved in formation of autophagosomal vacuoles (autophagosomes). |
| Immunogen | Peptide sequence around aa.5~10 (R-P-F-K-Q) derived from Human LC3A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ATG8E LC3 LC3A MAP1ALC3 MAP1BLC3 MAP1LC3A MAP1A/1B light chain 3 A microtubule-associated proteins 1A/1B light chain 3 autophagy-related ubiquitin-like modifier LC3 A autophagy-related protein LC3 A MAP1A/MAP1B LC3 A MAP1A/MAP1B light chain 3 A |
|---|---|
| Target | LC3A |
| Molecular Weight(MW) | 18, 16 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using LC3A antibody .
Application
Western blot analysis of extracts from Hela and 293 cells using LC3A antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
