Application
Western blot analysis using anti-LCK mAb against MOLT-4 (1), CCRF-CEM (2), CCRF-HSB-2 (3) and Jurkat (4) cell lysate.| Product Name | Lck antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [8E5F9, 4B2C7] to LckThis gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human Lck expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 8E5F9, 4B2C7 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | P56LCK YT16 PP58LCK LCK LSK |
|---|---|
| Target | Lck |
| Molecular Weight(MW) | 56 kDa (note) |
| Cellular Localization | Present in lipid rafts in an unactive form. |
Application
Western blot analysis using anti-LCK mAb against MOLT-4 (1), CCRF-CEM (2), CCRF-HSB-2 (3) and Jurkat (4) cell lysate.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
