Application
Sample(30 μg of whole cell lysate) A:H1299 7.5% SDS PAGE diluted at 1:500Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000| Product Name | Lck antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Lck (lymphocyte-specific protein tyrosine kinase)This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Lck. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, Sheep, BovineRat (97%), Zebrafish (85%), Xenopus laevis (86%), Pig (96%), Sheep (97%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LSK antibody YT16 antibody p56lck antibody pp58lck antibody LCK antibody t cell-specific protein-tyrosine kinase antibody tyrosine-protein kinase Lck antibody p56(LSTRA) protein-tyrosine kinase antibody proto-oncogene tyrosine-protein kinase LCK an |
|---|---|
| Target | Lck |
| Molecular Weight(MW) | 58 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Cell membrane; Lipid-anchor; Cytoplasmic side |
Application
Sample(30 μg of whole cell lysate) A:H1299 7.5% SDS PAGE diluted at 1:500
Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000| Positive Control | H1299 , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
