Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
LDH-B antibody detects LDHB protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. LDH-B antibody dilution: 1:200.Application
Immunofluorescence analysis of methanol-fixed HeLa, using LDH-B antibody at 1:50 dilution.| Product Name | LDH-B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LDH-B (lactate dehydrogenase B)This gene encodes an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on the X chromosome and on chromosome. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LDH-B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Dog, Pig, Rabbit, Chicken, Rhesus Monkey, Chimpanzee, BovineXenopus laevis (81%), Dog (100%), Pig (96%), Rabbit (100%), Chicken (87%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LDH-H antibody TRG-5 antibody LDHB antibody lactate dehydrogenase H chain antibody renal carcinoma antigen NY-REN-46 antibody L-lactate dehydrogenase B chain antibody LDH heart subunit antibody lactate dehydrogenase B antibody |
|---|---|
| Molecular Weight(MW) | 37 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
LDH-B antibody detects LDHB protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. LDH-B antibody dilution: 1:200.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using LDH-B antibody at 1:50 dilution.| Positive Control | U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
