Application
Flow cytometric analysis of Jurkat cells, using anti-LEMD3 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Anti-LEMD3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with LEMD3Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LEMD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LEMD3.Application
Flow cytometric analysis of Hela cells, using anti-LEMD3 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LEMD3 monoclonal antibody.| Product Name | LEMD3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3H1] to LEMD3Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. Antagonizes TGF-beta-induced cell proliferation arrest. |
| Immunogen | Full length human recombinant protein of human LEMD3 (NP_055134) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3H1 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 : |
|
| Species Reactivity | HumanDogMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MAN1 |
|---|---|
| Target | LEMD3 |
| Tissue Specificity | is specific for human LEMD3 (NP_055134). |
Application
Flow cytometric analysis of Jurkat cells, using anti-LEMD3 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Anti-LEMD3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with LEMD3
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LEMD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LEMD3.
Application
Flow cytometric analysis of Hela cells, using anti-LEMD3 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LEMD3 monoclonal antibody.| Application Notes | ICC/IF 1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
