Rabbit polyclonal to LeptinLeptin (also known as OB) is a 16-kD protein produced primarily in white adipose tissue, but also in brown fat and the placenta. It was first identified by positional cloning (ref 1). It is involved in regulating the food intake, energy expenditure, whole-body energy balance in rodents and humans (ref 2-5). In addition to its metabolic control role, leptin also plays important roles in reproduction, immunological response and neuro-endocrine signaling (ref 6).
Immunogen
Recombinant protein - full length mouse Leptin protein
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ICC/IFWB
For WB: Use at a dilution of 1:1,500 - 1:2,500. Optimal dilutions/concentrations should be determined by the researcher.:
Species Reactivity
Mouse
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
ob obese
Target
Leptin
Application
Application
Staining of formalin fixed paraffin embedded Trichuris muris infected mouse tissue with Rabbit anti Mouse leptin
Application
Application
Application
Western Blot detection of mouse OB protein in mOB transfected 293T cells
Application Notes
For WB: Use at a dilution of 1:1,500 - 1:2,500. Optimal dilutions/concentrations should be determined by the researcher.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.