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For WB: Use at a concentration of 1.0 μg/ml. Detects LexA expressed from a GAL1 promoter in S.cerevisiae extracts after induction with galactose. Reacts with LexA (approx. 27 kDa), by immunoblotting. A cross-reactive band of approximately 62 kDa may be observed in some S. cerevisiae strains. Optimal dilutions/concentrations should be determined by the end user:
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