Application
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGR5 antibody diluted at 1:500.Application
LGR5 antibody detects LGR5 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGR5 antibody diluted by 1:1000.Application
LGR5 antibody detects LGR5 protein by western blot analysis. SK-N-SH whole cell extracts and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGR5 antibody diluted by 1:500.Application
LGR5 antibody detects LGR5 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg HeLa membrane lysate/extract 7.5 % SDS-PAGE LGR5 antibody dilution: 1:2000Application
LGR5 antibody detects LGR5 protein by Western blot analysis. A. 30 µgHepG2 whole cell lysate/extract B. 30 µg HepG2 membrane fraction lysate/extract 7.5 % SDS-PAGE LGR5 antibody dilution: 1:1000| Product Name | LGR5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to LGR5 (leucine-rich repeat-containing G protein-coupled receptor 5)Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LGR5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Pig, BovineMouse (87%), Rat (89%), Xenopus laevis (81%), Pig (94%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FEX antibody GPR49 antibody GPR67 antibody GRP49 antibody HG38 antibody MGC117008 antibody LGR5 antibody G-protein coupled receptor HG38 antibody G-protein coupled receptor 67 antibody leucine-rich repeat-containing G protein-coupled receptor 5 an |
|---|---|
| Target | LGR5 |
| Molecular Weight(MW) | 100 kDa (note) (2) |
| Cellular Localization | Cell membrane; Multi-pass membrane protein |
Application
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGR5 antibody diluted at 1:500.
Application
LGR5 antibody detects LGR5 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGR5 antibody diluted by 1:1000.
Application
LGR5 antibody detects LGR5 protein by western blot analysis. SK-N-SH whole cell extracts and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGR5 antibody diluted by 1:500.
Application
LGR5 antibody detects LGR5 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg HeLa membrane lysate/extract 7.5 % SDS-PAGE LGR5 antibody dilution: 1:2000
Application
LGR5 antibody detects LGR5 protein by Western blot analysis. A. 30 µgHepG2 whole cell lysate/extract B. 30 µg HepG2 membrane fraction lysate/extract 7.5 % SDS-PAGE LGR5 antibody dilution: 1:1000| Positive Control | A431 , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS , HeLa , HeLa membrane , HepG2 , HepG2 membrane , SK-N-SH , SK-N-SH MF |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
