Application
LHX2 antibody detects LHX2 protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: LHX2 protein stained by LHX2 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .Application
LHX2 antibody detects LHX2 protein by western blot analysis. A 293T whole cell lysate/extract B A431 whole cell lysate/extract C HeLa D HepG2 whole cell lysate/extract 10% SDS-PAGE LHX2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
LHX2 antibody detects LHX2 protein by western blot analysis. A. Neuro2A whole cell lysate/extract B. GL261 whole cell lysate/extract C. C8D30 whole cell lysate/extract D. NIH-3T3 whole cell lysate/extract E. BCL-1 whole cell lysate/extract F. BCL-1 whole cell lysate/extract G. Raw264.7 whole cell lysate/extract H. C2C12 whole cell lysate/extract 10% SDS-PAGE LHX2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with LHX2 antibody diluted at 1:10000 (left panel) and 1:5000 (right panel). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | LHX2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LHX2 (LIM homeobox 2)This gene encodes a protein belonging to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator. The protein can recapitulate or rescue phenotypes in Drosophila caused by a related protein, suggesting conservation of function during evolution. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human LHX2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Chicken, Rhesus Monkey, BovineRat (95%), Pig (98%), Chicken (81%), Rhesus Monkey (98%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LIM homeobox 2 LH2 hLhx2 |
|---|---|
| Target | LHX2 |
| Cellular Localization | Nucleus |
Application
LHX2 antibody detects LHX2 protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: LHX2 protein stained by LHX2 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
LHX2 antibody detects LHX2 protein by western blot analysis. A 293T whole cell lysate/extract B A431 whole cell lysate/extract C HeLa D HepG2 whole cell lysate/extract 10% SDS-PAGE LHX2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
LHX2 antibody detects LHX2 protein by western blot analysis. A. Neuro2A whole cell lysate/extract B. GL261 whole cell lysate/extract C. C8D30 whole cell lysate/extract D. NIH-3T3 whole cell lysate/extract E. BCL-1 whole cell lysate/extract F. BCL-1 whole cell lysate/extract G. Raw264.7 whole cell lysate/extract H. C2C12 whole cell lysate/extract 10% SDS-PAGE LHX2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with LHX2 antibody diluted at 1:10000 (left panel) and 1:5000 (right panel). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , HepG2 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , mouse brain , mouse eye |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
