| Product Name | LHX3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to LHX3The pituitary gland releases hormones that regulate growth, lactation, homeostasis, the stress response, reproductive development and fitness as well as water metabolism. LHX3 is one of several transcription factors that have been implicated in pituitary development. This particular transcription factor is critical for motor neuron specification and the development of the anterior and intermediate lobes of the pituitary gland in mammals. The sequence of the human LHX3 gene has been identified and characterized by IU researchers. |
| Immunogen | C-terminal portion of mouse LHX3 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PIPWB |
| ICC: Use at a dilution of 1/100 - 1/250. IF: Use at an assay dependent dilution. Use a secondary antibody conjugated to biotin and then streptavidin-conjugated to fluorochrome. IHC-P: Use at a dilution of 1/125 - 1/500. IHC-Fr: 1:1500-1:3000. For whole mounts recommended fixative is MEMFA using a dilution of 1/2000 - 1/4000. IP: Use at a dilution of 1/100. WB: Use at a dilution of 1/1250 - 1/2500. Predicted molecular weight: 44 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRatFishFrog |
| Concentration | 0.5mg/ml |
| Purification | Protein A |
| Gene Synonyms | P-LIM Lhx3 PLIM mLim-3 mLim3 16871 Lim3 P LIM mLim 3 |
|---|---|
| Target | LHX3 |
| Tissue Specificity | recognizes LHX3 and does not appear to crossreact with gsh4 on tissue sections. |
| Cellular Localization | Nuclear |
| Application Notes | ICC: Use at a dilution of 1/100 - 1/250. IF: Use at an assay dependent dilution. Use a secondary antibody conjugated to biotin and then streptavidin-conjugated to fluorochrome. IHC-P: Use at a dilution of 1/125 - 1/500. IHC-Fr: 1:1500-1:3000. For whole mounts recommended fixative is MEMFA using a dilution of 1/2000 - 1/4000. IP: Use at a dilution of 1/100. WB: Use at a dilution of 1/1250 - 1/2500. Predicted molecular weight: 44 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: PBS. pH 7.2 |
