Application
LIM1 antibody detects LIM1 protein expression at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: LIM1 protein stained by LIM1 antibody diluted at 1:250. Red: SOX2, stained by SOX2 antibody [GT1876] diluted at 1:250. Blue: Fluoroshield with DAPI .Application
LIM1 antibody detects LIM1 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: LIM1 protein stained by LIM1 antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI .Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with LIM1 antibody diluted at 1:2000.Application
LIM1 antibody detects LIM1 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. LIM1 antibody diluted at 1:500.Application
LIM1 antibody detects LIM1 protein at nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. LIM1 antibody dilution: 1:500.| Product Name | LIM1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LIM1 (LIM homeobox 1)This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator and be involved in control of differentiation and development of neural and lymphoid cells. A similar protein in mice is an essential regulator of the vertebrate head organizer. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LIM1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | MouseRat |
| Predicted Reactivity | Human, Xenopus laevis, Chicken, Chimpanzee, BovineHuman (100%), Xenopus laevis (88%), Chicken (84%), Chimpanzee (100%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LIM-1 LHX1 LIM homeobox 1 |
|---|---|
| Target | LIM1 |
| Molecular Weight(MW) | 45 kDa (note) |
| Cellular Localization | Nucleus |
Application
LIM1 antibody detects LIM1 protein expression at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: LIM1 protein stained by LIM1 antibody diluted at 1:250. Red: SOX2, stained by SOX2 antibody [GT1876] diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
LIM1 antibody detects LIM1 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: LIM1 protein stained by LIM1 antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI .
Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with LIM1 antibody diluted at 1:2000.
Application
LIM1 antibody detects LIM1 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. LIM1 antibody diluted at 1:500.
Application
LIM1 antibody detects LIM1 protein at nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. LIM1 antibody dilution: 1:500.| Positive Control | mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
