Application
LIMD1 antibody [C2C3], C-term detects LIMD1 protein at nucleus on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. LIMD1 antibody [C2C3], C-term diluted at 1:500.Application
LIMD1 antibody [C2C3], C-term detects LIMD1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LIMD1 protein stained by LIMD1 antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using LIMD1 antibody at 1:300 dilution.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample(30 µg whole cell lysate) A:293T 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | LIMD1 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LIMD1 (LIM domains containing 1)Transcriptional regulator that inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. May act as a tumor suppressor by inhibiting cell proliferation. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human LIMD1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (98%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LIMD1 antibody LIM domain-containing protein 1 antibody LIM domains containing 1 antibody |
|---|---|
| Molecular Weight(MW) | 72 kDa (note) |
Application
LIMD1 antibody [C2C3], C-term detects LIMD1 protein at nucleus on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. LIMD1 antibody [C2C3], C-term diluted at 1:500.
Application
LIMD1 antibody [C2C3], C-term detects LIMD1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LIMD1 protein stained by LIMD1 antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using LIMD1 antibody at 1:300 dilution.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample(30 µg whole cell lysate) A:293T 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
