Application
Western blot analysis of LIMK1/2 (pT508/505) expression in HeLa UV-treated (A); Raw264.7 UV-treated (B); A431 (C); NIH3T3 (D); PC12 (E) whole cell lysates.| Product Name | LIMK1 (phospho Thr508) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LIMK1 (phospho Thr508)There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Feb 2011] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human LIMK1/2. The exact sequence is proprietary. |
| Modification | p-Thr508 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LIMK , LIMK1 , LIM domain kinase 1 , LIMK-1 |
|---|---|
| Target | LIMK1 (phospho Thr508) |
| Tissue Specificity | Recognizes endogenous levels of LIMK1/2 (pT508/505) protein. |
Application
Western blot analysis of LIMK1/2 (pT508/505) expression in HeLa UV-treated (A); Raw264.7 UV-treated (B); A431 (C); NIH3T3 (D); PC12 (E) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
