| Product Name | LIMK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to LIMK1LIM kinases (LIMK1 and LIMK2) are serine/threnine kinases that have two zinc finger motifs, known as LIM motifs in their N-terminal regulatory domain. LIM kinases are involved in actin cytoskeleton regulation through Rho-family GTPases and downstream kinases PAKs and ROCK. PAK1 and ROCK phosphorylate LIMK1 and LIMK2, which increases the activity of the kinases. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the N-terminus of Cofilin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| Western blotting (1-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 70 kDa LIMK1 protein. It does not cross-react with LIMK2. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LIM kinases, LIMK1, LIMK-1, LIMK 1 |
|---|---|
| Target | LIMK1 |
| Tissue Specificity | This antibody is specific for LIMK1. |
| Application Notes | Western blotting (1-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 70 kDa LIMK1 protein. It does not cross-react with LIMK2. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 50% glycerol, 1% BSA, 0.02% thimerosal |
