Application
Immunohistochemistry of LIMP2 in human skeletal muscle with LIMP2 antibody at 2.5 ug/ml.Application
Western blot analysis of LIMP2 in human skeletal muscle tissue lysate with LIMP2 antibody at 1 ug/ml in (A) the absence and (B) presence of blocking peptide.Application
Immunofluorescence of LIMP2 in Human Skeletal Muscle cells with LIMP2 antibody at 20 ug/mL.| Product Name | LIMP II antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to LIMP IIThe lysosomal integral membrane protein 2 (LIMP2) is a heavily glycosylated type III transmembrane protein, the majority of which exists in the lumen of the lysosome and a cytoplasmic domain of approximately 20 amino acids. A deficiency of LIMP2 in mice causes uretic pelvic junction obstruction, deafness, and peripheral neuropathy associated with impaired vesicular trafficking and distribution of apically expressed proteins. More recently, LIMP2 was shown to act as a receptor to bind b-glucocerebrosidase, the enzyme defective in Gaucher disease, a lysosomal storage disorder. LIMP2-deficient mice showed missorted as well as secreted b-glucocerebrosidase, suggesting that LIMP2 also functions as the mannose-6-phosphate-independent trafficking receptor. Despite its predicted molecular weight, LIMP2 runs at approximately 80 – 85 kDa in SDS-PAGE. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD36L2 Lysosomal integral membrane protein 2 SR-B2 SRB2 Scavenger receptor class B member2 Lysosomal integral membrane protein2 SCARB2 LIMP2 Scavenger receptor class B member 2 SR B2 |
|---|---|
| Target | LIMP2 |
| Tissue Specificity | This antibody is specific for LIMP2 |
Application
Immunohistochemistry of LIMP2 in human skeletal muscle with LIMP2 antibody at 2.5 ug/ml.
Application
Western blot analysis of LIMP2 in human skeletal muscle tissue lysate with LIMP2 antibody at 1 ug/ml in (A) the absence and (B) presence of blocking peptide.
Application
Immunofluorescence of LIMP2 in Human Skeletal Muscle cells with LIMP2 antibody at 20 ug/mL.| Positive Control | Blocking Peptide: Cat.No. GTX85033-PEP - LIMP2 Peptide Lysates: Cat. No. GTX15370 - Human Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Antibody is supplied in PBS containing 0.02% sodium azide |
