Application
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with LIN28B antibody diluted at 1:1000.Application
LIN28B antibody detects LIN28B protein at cytoplasm and nucleus in human colon by immunohistochemical analysis. Sample: Paraffin-embedded human colon. LIN28B antibody diluted at 1:500.Application
LIN28B antibody detects LIN28B protein at cytoplasm and nucleus in human ovary by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. LIN28B antibody diluted at 1:500.| Product Name | LIN28B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LIN28B (lin-28 homolog B (C. elegans))Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LIN28B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Sheep (82%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | lin-28 homolog B (C. elegans) , CSDD2 |
|---|---|
| Target | LIN28B |
| Molecular Weight(MW) | 27 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with LIN28B antibody diluted at 1:1000.
Application
LIN28B antibody detects LIN28B protein at cytoplasm and nucleus in human colon by immunohistochemical analysis. Sample: Paraffin-embedded human colon. LIN28B antibody diluted at 1:500.
Application
LIN28B antibody detects LIN28B protein at cytoplasm and nucleus in human ovary by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. LIN28B antibody diluted at 1:500.| Positive Control | Huh7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
