Application
LIN7A antibody [N1C3] detects LIN7A protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: LIN7A protein stained by LIN7A antibody [N1C3] diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Sample (30 µg of whole cell lysate) A: MCF-7 12% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using LIN7A antibody at 1:250 dilution.| Product Name | LIN7A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LIN7A (lin-7 homolog A (C. elegans))Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LIN7A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, BovineMouse (100%), Rat (100%), Zebrafish (97%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LIN-7A antibody, LIN7 antibody, MALS-1 antibody, MGC148143 antibody, TIP-33 antibody, VELI1 antibody, LIN7A antibody, vertebrate LIN7 homolog 1 antibody, veli-1 antibody, tax interaction protein 33 antibody, protein lin-7 homolog A antibody, mammalian LIN- |
|---|---|
| Molecular Weight(MW) | 26 kDa (note) (2) |
| Cellular Localization | Cell membrane; Peripheral membrane protein , Basolateral cell membrane; Peripheral membrane protein , Cell junction (By similarity) , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density; Peripheral membrane protein (By similarity) |
Application
LIN7A antibody [N1C3] detects LIN7A protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: LIN7A protein stained by LIN7A antibody [N1C3] diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Sample (30 µg of whole cell lysate) A: MCF-7 12% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using LIN7A antibody at 1:250 dilution.| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
