Application
Sample (30 µg of whole cell lysate) A: MCF-7 7.5% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | LINGO1 antibody [C1C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Lingo-1 (leucine rich repeat and Ig domain containing 1)Functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation responsible for some inhibition of axonal regeneration by myelin-associated factors. Is also an important negative regulator of oligodentrocyte differentiation and axonal myelination. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Lingo-1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, ChickenMouse (99%), Rat (99%), Chicken (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ14594 antibody, LERN1 antibody, LRRN6A antibody, MGC17422 antibody, UNQ201 antibody, LINGO1 antibody, leucine rich repeat neuronal 6A antibody, leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1 antibody, l |
|---|---|
| Molecular Weight(MW) | 70 kDa (note) (13) |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
Application
Sample (30 µg of whole cell lysate) A: MCF-7 7.5% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
