Application
Liprin alpha 1 antibody [N1N2], N-term detects PPFIA1 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract 5 % SDS-PAGE Liprin alpha 1 antibody [N1N2], N-term dilution: 1:1000Application
Sample (30 ug of whole cell lysate) A: JurKat 5% SDS PAGE diluted at 1:10000Application
Liprin alpha 1 antibody [N1N2], N-term detects Liprin alpha 1 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: Liprin alpha 1 protein stained by Liprin alpha 1 antibody [N1N2], N-term diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Liprin alpha 1 antibody detects Liprin alpha 1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Liprin alpha 1 antibody diluted by 1:1000.Application
Immunoprecipitation of Liprin alpha 1 protein from A431 whole cell extracts using 5 μg of Liprin alpha 1 antibody [N1N2], N-term . Western blot analysis was performed using Liprin alpha 1 antibody [N1N2], N-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | Liprin alpha 1 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Liprin alpha 1 (protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1)The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. This protein binds to the intracellular membrane-distal phosphatase domain of tyrosine phosphatase LAR, and appears to localize LAR to cell focal adhesions. This interaction may regulate the disassembly of focal adhesion and thus help orchestrate cell-matrix interactions. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Liprin alpha 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, BovineRat (95%), Zebrafish (81%), Xenopus laevis (86%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ41337 antibody, FLJ42630 antibody, FLJ43474 antibody, LIP.1 antibody, LIP1 antibody, LIPRIN antibody, MGC26800 antibody, PPFIA1 antibody, LAR-interacting protein 1 antibody, PTPRF-interacting protein alpha-1 antibody, Liprin-alpha1 antibody, liprin-alph |
|---|---|
| Molecular Weight(MW) | 136 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
Liprin alpha 1 antibody [N1N2], N-term detects PPFIA1 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract 5 % SDS-PAGE Liprin alpha 1 antibody [N1N2], N-term dilution: 1:1000
Application
Sample (30 ug of whole cell lysate) A: JurKat 5% SDS PAGE diluted at 1:10000
Application
Liprin alpha 1 antibody [N1N2], N-term detects Liprin alpha 1 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: Liprin alpha 1 protein stained by Liprin alpha 1 antibody [N1N2], N-term diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Liprin alpha 1 antibody detects Liprin alpha 1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Liprin alpha 1 antibody diluted by 1:1000.
Application
Immunoprecipitation of Liprin alpha 1 protein from A431 whole cell extracts using 5 μg of Liprin alpha 1 antibody [N1N2], N-term . Western blot analysis was performed using Liprin alpha 1 antibody [N1N2], N-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | 293T , A431 , HeLa , HepG2 , JurKat , Neuro2A |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
