Application
Livin antibody detects BIRC7 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract 12 % SDS-PAGE Livin antibody dilution: 1:500Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ML-IAP antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using ML-IAP antibody at 1:100 dilution.| Product Name | Livin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Livin (baculoviral IAP repeat containing 7)The protein encoded by this gene is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Two transcript variants encoding different isoforms have been found for this gene. The two isoforms have different antiapoptotic properties, with isoform alpha protecting cells from apoptosis induced by staurosporine and isoform b protecting cells from apoptosis induced by etoposide. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Livin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KIAP antibody, LIVIN antibody, ML-IAP antibody, MLIAP antibody, RNF50 antibody, BIRC7 antibody, melanoma inhibitor of apoptosis protein antibody, livin inhibitor of apoptosis antibody, kidney inhibitor of apoptosis protein antibody, RING finger protein 50 |
|---|---|
| Molecular Weight(MW) | 33 kDa (note) (3) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Livin antibody detects BIRC7 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract 12 % SDS-PAGE Livin antibody dilution: 1:500
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ML-IAP antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using ML-IAP antibody at 1:100 dilution.| Positive Control | 293T , A431 , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
