Application
Immunohistochemical staining of Livin in human small intestine with Livin antibody at 5μg/ml.Application
Western blot analysis of Livin expression in human Raji cell lysate with Livin antibody at 0.5μg/ml. Positive control: Raji cell lysate| Product Name | Livin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to LivinApoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel member in the IAP protein family was recently identified and designated Livin and KIAP for kidney IAP. Livin / XIAP contains a single baculoviral IAP repeat (BIR) domain and a RING finger domain and has two isoforms termed Livin alpha and Livin beta. Transfection of Livin in cells resulted in protection from apoptosis induced by FADD, BAX, RIP, RIP 3 and DR6. Livin has direct interaction with several caspases including caspase 3, 7, and 9. Livin inhibits the activation of caspase 9 induced by Apaf 1, cytochrome c, and dATP. The two isoforms of Livin appear to have different functions and tissue distributions. |
| Immunogen | Synthetic peptide: CRAPVRSRVRTFLS, corresponding to amino acids 264 - 280 (short form) or 281 - 298 (long form) of Human Livin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: Use at a concentration of 2 - 4 μg/ml. Detects bands of approximately 30 kDa (possibly short form) + 33 kDa (long form). Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 605737, Q96CA5, LIVIN, 79444, ML-IAP, MLIAP, BIRC7, KIAP, RNF50, ML IAP |
|---|---|
| Target | Livin |
| Tissue Specificity | Detects both the alpha and beta (short and long) isoforms of Livin. |
Application
Immunohistochemical staining of Livin in human small intestine with Livin antibody at 5μg/ml.
Application
Western blot analysis of Livin expression in human Raji cell lysate with Livin antibody at 0.5μg/ml. Positive control: Raji cell lysate| Application Notes | WB: Use at a concentration of 2 - 4 μg/ml. Detects bands of approximately 30 kDa (possibly short form) + 33 kDa (long form). Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide |
